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Figure 4


Fig. 4. Inactivation of precocious heterochronic genes prevents the supernumerary molt of let-7 mir-84 mutants. Synchronized populations of L1 larvae were fed bacteria expressing dsRNA corresponding to the indicated genes and cultivated at 25°C. Animals in the P0 generation were scored for fluorescence from mlt-10p::gfp-pest 56 hours later and then for viability the next day. To score F1 animals, progeny were collected, synchronized as L1 larvae, and then fed the appropriate bacterial clone for an additional 55 hours.





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