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Figure 5


Fig. 5. Inactivation of nhr-23 or nhr-25 blocks the supernumerary molt of let-7 mir-84 mutants. (A,B) Synchronized populations of let-7(mg279) mir-84(tm1304) animals were cultured until the fourth larval stage at 20°C. Individuals were then fed bacteria expressing dsRNA and observed several times over the next 2 days. The combined results of three independent experiments are shown. Asterisks indicate a significant difference from the control sample (P ≤0.001, chi-square test). (A) Prevalence of detectable fluorescence from mlt-10p::gfp-pest. (B) Prevalence of death. (C) Fluorescence from nhr-23::gfp in the nuclei of epithelial cells near the head of let-7 mir-84 or wild-type adults.





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