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Fig. 5. Segmental clock and number of somites in Shisa2 morphants.
Whole-mount in situ hybridization of wild-type embryos (A-C) or embryos
unilaterally injected with MO1 (D-F) at stage 18 (posterior view,
dorsal toward the top). The phase of cyclic ESR9 expressions was
determined according to Li et al. (Li et
al., 2003). (A,D) Phase I. (B,E) Phase II. (C,F) Phase III.
Arrowheads in D-F indicate the anterior border of ESR9 expression.
The phases of the ESR9 expression between the MO-injected and
uninjected side were symmetrical (5mis, n=14/17; MO1,
n=24/24; MO2, n=25/29); however, the expression domain
expanded anteriorly in the Shisa2-depleted side (5mis, n=0/19; MO1,
n=18/24; MO2, n=22/29). (G-L) Longitudinal sections
of the embryos unilaterally depleted Shisa2 stained with hematoxylene and
eosin at the 6-9 somite stage. MO-injected sides are indicated on the left.
(G,I,K) Sections of the level at the first somite. (H,J,L) Sections in a more
posterior region of G, I and K. The positions of the last formed somites are
displaced anteriorly in the Shisa2-depleted sides, for a distance of one (J;
5mis, n=2/11; MO1, n=10/16; MO2, n=6/15) or two (L;
5mis, n=0/11; MO1, n=2/16; MO2, n=4/15)
somites.
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