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Figure 6


Fig. 6. Bchs localization in presynaptic endings. (A) Bchs immunoreactivity (red) within the third-instar larval brain and nerve cord is enriched in the synaptic neuropil, marked with Synaptotagmin1 immunoreactivity (green). Bchs immunoreactivity is undetectable in bchs12. (B) When expressed in the adult ellipsoid body, HA-tagged Bchs (red) accumulates in the axon terminals of EB neurons, while transmembrane mCD8::GFP (green) distributes evenly throughout these neurons. Stars denote EB neuron presynaptic terminals, arrowheads point to EB neuron dendrites, arrows point to EB cell bodies. (C) In a confocal stack of images from a third-instar NMJ (muscle 6/7), Bchs immunoreactivity (red) is enriched in synaptic boutons. The neuronal membrane is outlined by anti-HRP (green). (D) In a single confocal section, Bchs puncta (red) are visible within the HRP-labeled (green) neuronal ending. The specificity of this immunoreactivity is confirmed by its absence in bchs58. Scale bars: 20 µm in C; 5 µm in D. EB, ellipsoid body.





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