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Fig. 1. pol pll1 mutant tissue phenocopies wus mutants.
(A,B) pol pll1 seedlings were not viable because of
major defects in the basal embryo and vascular development compared with
wild-type siblings (C,D). (B,D) Higher magnification of A and C,
respectively. (E) pol pll1 apical tissue can be rescued by
grafting to the hypocotyl and root of a wild-type seedling. (F-T)
Grafted pol pll1 in the ER+ (F,K,P),
er- (G,L,Q) and clv3-2 (H,M,R) backgrounds
exhibited meristem termination similar to wus-1 mutants (I,N,S)
during vegetative (F-J), inflorescence (K-O) and flower (P-T) development.
(J,O,T) Wild-type Ler shown as a control. Similar meristem
termination, albeit with reduced penetrance and expressivity, was observed in
pol/pol pll1/+ plants (U,V), pol
mutants expressing antisense PLL1 (W,X), and pol
pll1 mutants incompletely rescued by PPLL1:PLL1
expression (Y). Arrows indicate junction of cotyledon vascular elements
(B,D) or graft junction (E). Asterisks indicate flowers that developed with
reduced or absent gynoecia. Insets in V and X show flowers with reduced or
absent gynoecia, respectively, at higher magnifications.