DEV02675 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. wg (but not mirr) expression is regulated by JAK/STAT signaling. (A,B) A third instar wg-lacZ (A) or wg-lacZ; stat92E^85C9 M⁺ (B) eye disc. Heterozygous tissue (green), wg-lacZ (anti-β-Gal, red) and Elav (blue). wg is ectopically expressed throughout the entire stat92E^85C9 M⁺ eye disc. (C) A third instar eye disc carrying ey>upd and wg-lacZ (ey>upd; wg-lacZ). wg-lacZ (anti-β-Gal, red) and Elav (blue). Ectopic upd expression completely represses dorsal wg, allowing precocious photoreceptor differentiation from the lateral margin. In this disc, photoreceptors have developed throughout the eye disc, including regions that would otherwise give rise to head cuticle. (D,E) A third instar eye disc carrying mirr-lacZ in a wild-type background (mirr-lacZ; D) or in a stat92E^85C9 Minute background (mirr-lacZ, stat92E^85C9 M⁺; E). Heterozygous tissue (green), mirr-lacZ (anti-β-Gal, red) and Elav (blue). mirr-lacZ expression is not altered in stat92E^85C9 M⁺ discs. (F,G) Adult eyes carrying mirr-lacZ in a wild-type background (mirr-lacZ; F) or in a stat92E^85C9 Minute background (mirr-lacZ, stat92E^85C9 M⁺; G). Orange ommatidia represent mirr-expressing (dorsal) cells and white ommatidia do not express mirr (ventral cells). stat92E^85C9 M⁺ adult eyes are frequently composed exclusively of non-mirr-expressing ventral tissue (G).