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Fig. 1. Upd acts in the early eye disc to promote regional specification.
(A,B) upd>GFP expression (green) in first (A) and
second (B) instar eye discs; Dlg marks the cell outline (red).
(C,D) upd mRNA in situ hybridization analysis.
upd is transcribed at the posterior midline in late second/early
third (C), but not in mid-third (D) instar eye discs. (E-H) Expression
of 10XSTAT92E-GFP (abbreviated 10XSTAT-GFP; green), Dlg
(blue) and dpp-lacZ (anti-ß-Gal, red) (E,F) or wg-lacZ
(anti-ß-Gal, red) (G,H). 10XSTAT-GFP is expressed in the
posterior domain of first (E,G) and second (F,H) instar eye discs. This
expression partially overlaps with dpp (E,F) and abuts wg
(G,H). (I,J) Scanning electronic micrographs of wild-type (I)
and ey-GAL4, UAS-upd (ey>upd) (J) adult heads, dorsal
view. Overexpression of upd results in the expansion of the dorsal
eye domain into the lateral head cuticle, reducing the inter-eye distance
(arrow). ey>upd animals also have patterning defects in both the
eye and the head cuticle. In all images, eye discs are oriented with anterior
towards the left and dorsal upwards.
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