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Fig. 5. Stat92E regulates wg through a small enhancer in the wg 3' cis genomic region. (A) Schematic representation of 20 kb of the wg gene, from just 5' of the start site, through untranslated and coding exons (turquoise and red boxes, respectively), and including 9 kb of 3' cis genomic region. wg2.11Z is a 263 bp enhancer from the wg 3' cis genomic region that is sufficient to recapitulate wg expression in the dorsal eye (B). This 20 kb region contains only one putative Stat92E binding site (TTC(N)₃GAA; red arrow), which resides downstream of the wg2.11Z enhancer. (B-D) X-gal staining of wg2.11Z (blue). Third instar eye discs carrying the wg2.11Z reporter (B) and a stat92E^85C9 chromosome in a non-Minute (C) or Minute (D) background. Clones were generated using ey-flp. wg is expanded in stat92E^85C9 non-Minute (C) and Minute (D) clones in a manner similar to wg-lacZ (see Fig. 3E,F). Outgrowths of tissue containing ectopic wg2.11Z are evident during larval stages (C, arrow). (E,E') A third instar eye disc carrying the wg2.11Z reporter (anti-ß-Gal, red) and hop flip-out clones (Actin>>GAL4; UAS-hop/wg2.11Z; green). hop flip-out clones autonomously repress wg2.11Z expression. (E) Merge; (E') wg2.11Z single-channel.

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