DEV02659 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. mutant cells in the developing eye imaginal disc do not exhibit obvious defects in lipid-droplet accumulation. An eye imaginal disc in which mutant (GFP-negative) clones have been induced using the eyflp/FRT recombination system (Newsome et al., 2000). The disc was stained with Nile Red. (A-C) The eye disc is distorted because of the overgrowth in mutant tissue. Some Nile Red stain accumulates adjacent to the edge of the disc. The arrow in C marks the approximate location of the morphogenetic furrow. Differentiating photoreceptors and other accessory cells of the eye are found more posteriorly (to the right of the furrow). (D-F and G-I) show higher magnification views of parts of the same disc. The regions examined are marked in A by squares (top square D-F; bottom square G-I). The posterior cells in D-F have started to form regular ommatidial arrays. (G-I) shows a more apical view in a less-differentiated region of the eye. Sporadic apical lipid droplets are found in both mutant and wild-type regions.

• Supplemental Figure 2 -

  Fig. S2. Nuclear integrity is preserved in stage-11 mutant nurse cells. (A-D) Hoechst-stained nuclei (blue; stained using standard methods; D) in a stage-11 egg chamber containing an anterior mutant nurse-cell clone (non-GFP labeled (B) on left; oocyte is almost entirely to the right of this image). Both normal and mutant cells contain intact nuclei. At this stage, nurse-cell cytoplasm has started to be pumped into the oocyte, so some lipid droplets may have been redistributed between cells. However, there are still a considerable number of enlarged Nile Red-stained lipid droplets in mutant cells (C). The mutant cell at the bottom of the panel (arrow) contains very large lipid droplets that are not visible in this plane of focus. Scale bar: 40 μm.