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Fig. 1. Increased IIS produces lipid-droplet accumulation defects in nurse
cells. (A,B) Nile Red-positive small neutral lipid droplets
(red) accumulate in wild-type nurse cells, particularly in a perinuclear
region (arrowheads in B), which appears to co-localize with the endoplasmic
reticulum (Teixeira et al.,
2003). By contrast, non-GFP-labelled (not green) Pten
mutant cells (D-I) contain large aggregated lipid droplets of up to 15
µm diameter (arrowheads in E,H; shown at high magnification in F,I) and
contain far fewer small droplets. (C) This phenotype is completely
suppressed in Pten mutant animals rescued by a specific combination
of Akt alleles (Stocker et al.,
2002). (J-O) In some egg chambers, overexpression of
Dp110 in nurse cells induces sporadic formation of superficial,
elongated lipid-containing structures close to the plasma membrane (arrowheads
in K,N). The profile of these cells is outlined by intense GFP expression in
overlying somatic stretched follicular cells (green in L,O; see also deeper
section in Fig. 3C). Scale
bars: 40 µm in A-C,F,I; 40 µm in E for D,E; 40 µm in G for G,H; 40
µm in J for J,K,L; and 40 µm in M for M,N.O.
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