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Fig. 2. Morphological abnormality in the submandibular duct of
Cp2l1tra/tra mice. (A) Schematic representation
of the rodent SMG. (B) Morphological abnormalities observed in adult
male and female Cp2l1tra/tra mice. (C-E) In situ
hybridization analysis of the adult SMG. Nuclei were counterstained with
nuclear Fast Red (red). The GCT marker ß-NGF (C) and the basal
cell marker Sgn1 (D) were not detected in either male or female
Cp2l1tra/tra mice. Mist1 expression was normal in
the mutants (E). (F) Hematoxylin/eosin-stained sections of wild-type
(+/+) and Cp2l1tra/tra (tra/tra) fetuses at E18. Although
the numbers of cells surrounding the lumen of Cp2l1tra/tra
was almost equal to that of Cp2l1+/tra mice [mean cell
number ± standard deviation, 9.0±1.7 (n=25) versus
8.8±1.8 (n=20) in intralobular ducts and 10.8±1.7
(n=20) versus 10.2±1.8 (n=16) in the excretory
ducts], the lumen of Cp2l1tra/tra was significantly wider
in Cp2l1+/tra than in Cp2l1tra/tra
mice [mean diameter ± standard deviation, 2.69±0.83
(n=25) versus 3.68±1.16 µm (n=20) in the
intralobular ducts (P<0.005) and 5.34±2.77 (n=20)
and 7.2±2.0 µm (n=16) in the excretory ducts
(P<0.05)]. (G,H) Electron microscopic observations
of the duct. Apical structures of luminal cells (arrowheads in G and arrows in
H) did not develop sufficiently in Cp2l1tra/tra compared
with Cp2l1+/tra mice. (I) Immunohistological
staining of CP2L1 and keratins in the SMG at birth. Anti-CP2L1
immunoreactivity was found only in the duct cells (left panels). Detection of
cytokeratins (right panels) on the luminal surface in both the ducts (white
arrows) and acini (asterisks) in Cp2l1+/tra mice. In
Cp2l1tra/tra mice, cytokeratins were detected in acini but
not in the ducts. Scale bars: 100 µm in B-E; 10 µm in F; 7 µm in G; 1
µm in H; 50 µm in I. BC, basal cell; EXD, excretory duct; ID,
intercalated duct; STD, striated duct.
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