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Fig. 4. CP2L1 expression during kidney development. (A) Detection of
CP2L1 protein (green) in the ureteric epithelium (CD; arrowhead), identified
by expression of cytokeratin (red), and at the distal end of the S-shaped body
(arrow) at birth. DNA was stained with TOPRO3 (blue). (B) CP2L1
(lacZ) was detected strongly in the CNT, DCT and TAL and weakly in
the DL and PCT at the primitive loop nephron at E16. (C-F)
Identification of CP2L1 expression domains at birth by co-labeling by
LacZ staining (blue) and in situ hybridization (orange). Insets:
higher magnification of boxed regions. CP2L1 (LacZ) was strongly
coexpressed with Ncx1 (C) and modestly or weakly with Ncc
(D) at the immature loop nephron in deeper regions of the cortex. Few CP2L1
were detected with Nkcc2 (E) (arrowheads) and Slc34a1 (F).
Note that RNA localization of several genes, including Slc34a1, Ncx1
and Ncc (C,D,F), was detected only in the nucleus by in situ
hybridization used in this analysis. (G) Double-labeling of CP2L1
(lacZ) and Umod (orange) in the medulla. The expression of
CP2L1 was weakly detected in Umod-positive TAL at P0 (blue dots
indicated by black arrowheads in the upper inset) but disappeared in
adulthood. (H) Expression of CP2L1 (lacZ) at P0 and P10. The
expression was lost at P10 in the papillary region (white arrows). (I)
Summary of CP2L1 expression. The expression shifts during the course of
nephron maturation. Scale bar: 20 µm in A; 50 µm in C-G; 200 µm in
H.
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