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Fig. 6. Differentiation defects in the kidney of
Cp2l1tra/tra mice. (A-K) In situ hybridization
analysis of the kidney at birth. Expression of IC markers Atp6b1 (A)
and Slc4a1 (B) was lost in Cp2l1tra/tra mice.
Expression of keratin 7 was lower in the whole CD of
Cp2l1tra/tra mice at E18 (C,E) and in the cortical CD
(D,F; arrowheads) of newborn Cp2l1tra/tra mice. By
contrast, keratin 19 was more strongly expressed in the inner medullary CD of
Cp2l1tra/tra mice than Cp2l1+/tra mice
at birth (G). Expression of Gk-6 and Fxyd2c (orange) in DCT
and CNT overlapped with that of Cp2l1 (lacZ staining; blue)
(H). Expression of Gk-6 and Fxyd2c was not detected in
Cp2l1tra/tra mice at E18 (I,J). Expression of
Clcnkb was also absent in the distal tubules of
Cp2l1tra/tra mice (K). (L,M) Quantification
of mRNA expression by quantitative RT-PCR in isolated E14 kidney organ
cultures (L) or in dissected kidneys at different developmental stages (M).
The experiments were repeated at least twice. *P<0.005,
P<0.05. (N) Comparison of gene
expression between the kidney and the salivary glands. Analogous changes in
gene expression were observed in these organs in
Cp2l1tra/tra mice. Changes are indicated with arrows, and
a battery of genes commonly expressed in the developmental programs of these
organs is shown in color. Scale bars: 200 µm in A,C,D,G,I,K; 100 µm in
B,E,F,H,J.
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