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Figure 1


Fig. 1. olt and ord genes are required for migration of nVII motor neurons. (A-I) Morphology and Isl1-GFP expression of wild-type (A,D,G), MZ-oltrw689 (B,E,H) and MZ-ordrw71 (C,F,I) Isl1-GFP embryos at 48 hpf (A-F) and 30 hpf (G-I). In wild-type embryos (D,G), the nVII motor neurons are located in r6 (arrows). By contrast, in olt (E,H) and ord (F,I) embryos, most or all of the neurons are located in r4 (arrows). In ord embryos (F,I), some neurons migrate into r5 (arrowheads). (A-C,G-I) Lateral views; anterior facing left. (D-F) Dorsal views; anterior at the top. (A-F) Images obtained using a dissecting microscope. (G-I) Composite images of serial optical sections obtained by confocal microscopy. Va, anterior trigeminal nuclei; Vp, posterior trigeminal nuclei; VII, facial nucleus; X, vagus nucleus. Scale bars: 50 µm. The images in A,C,D,F,G,I were previously published in Wada et al. (Wada et al., 2005).





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