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Fig. 3. Identification of the ord gene as zebrafish celsr2
gene. (A) Genetic map of the ord locus. (B) Genomic
structure of the zebrafish celsr2 gene. The nucleotide substitution
resulting from each mutation is indicated. (C) Schematic drawings of
the mouse and zebrafish Celsr2 proteins. Amino acid sequence similarity (%) is
shown for each domain. The amino acid substitution resulting from each
mutation is indicated. (D) The phylogenetic tree for celsrfamily genes.
(E-G) Lateral views of wild-type embryos reacted with RNA probes for
celsr2 (E), celsr1a (F) and celsr1b (G) at 24 hpf.
(E',F',G') Cross sections at r5 for
each embryo, E-G, respectively. (H-K) A wild-type Isl1-GFP embryo
injected with celsr2-MO (J) shows incomplete disruption of the
neuronal migration as observed in an ord embryo (I). (K) An
ord mutant embryo injected with celsr1a/1b-MOs shows
complete loss of migration of the nVII motor neurons. The embryos are shown in
dorsal view and the images are composite stacks of serial optical sections.
(L) The neuronal migration phenotype in each experiment was scored as
follows: r4, complete loss of migration, as shown in K; r5, partial disruption
of migration, as shown in I; and r6, normal migration, as shown in H. Scale
bars: 50 µm.
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