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Figure 1


Fig. 1. hap2 disrupts pollen tube guidance. (A) In vitro grown hap2/HAP2 pollen tubes stained to reveal GUS expression in hap2 pollen tubes. (B) Quantitative analysis of ovule targeting. ms1 pistils were hand-pollinated with hap2/HAP2 or control pollen heterozygous for LAT52:GUS. The number of ovules receiving GUS activity from the pollen tube was counted for each and is plotted as a percentage (±s.d.) of the total number of ovules. (C-F) ms1 pistils stained for GUS activity 14 hours after pollination. (C) An ovule that has received a GUS+ control pollen tube. (D) An ovule that has received a GUS+ hap2 pollen tube. GUS is released into synergids from tubes that successfully enter the micropyle and burst (arrowheads in C and D). (E,F) hap2 pollen tube tips that fail to enter the micropyle (arrows). m, micropyle; f, funiculus. Scale bars: 40 µm in A; 20 µm in C-F.





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