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Fig. 2. Cell death in L/Ser-mutant cells is p53-independent and
caspase-dependent. (A-B') Overexpression of p53 (A) in the
wild-type eye using ey-GAL4 (ey>p53) results in a small
eye and, in the L2/+-mutant eye (L2/+;
ey>p53; B,B'), does not affect the loss-of-ventral-eye
phenotype. Dashed line marks the lost boundary of the eye field.
(C-D') Blocking caspase-dependent cell death by overexpression of
baculovirus P35 in the wild-type eye (C) does not affect eye size whereas, in
the L2/+-mutant background (L2/+;
ey>p53), this can significantly rescue the loss-of-ventral-eye
phenotype in the eye imaginal disc (D) and adult eye (D').
(E-H') Overexpression of diap1 (ey>diap1; E)
or a reduction in the levels of the Hid-Reaper-Grim complex by using
deficiency H99 (G) in wild-type eye does not affect eye size, whereas, in
L2/+-mutant backgrounds (F,F',H,H'), these can
rescue the loss-of-ventral-eye phenotype. (I) Overexpression of
dominant-negative Ser (ey>SerDN)
results in loss of ventral eye (Singh and
Choi, 2003). Dashed line marks the lost boundary of the eye field.
(J,J') Blocking caspase-dependent cell death can
significantly rescue the loss-of-ventral-eye phenotype of dominant-negative
Ser overexpression (ey>SerDN+P35), as
seen in eye disc (J) and adult eye (J'). Markers for immunostaining are
shown in color labels.
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