|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. L is involved in the repression of wg during second
larval instar. (A-C) Abolishing wg function in different
time windows using a temperature-sensitive wgIL114 allele
(details in Materials and methods) shows that second instar of larval
development (L2) is crucial for L-Wg interaction. (B) When wg
function was abolished during first or third instar, there was no significant
rescue of the L-mutant phenotype. (C) When wg function was
abolished during second instar, loss-of-ventral-eye phenotype of
L2/+ mutants was significantly rescued to near that of
wild-type eye. (D) In second instar wild-type larval eye disc,
wg-lacZ is expressed strongly on the dorsal margin. (E) In the
L2/+-mutant background, a strong induction of
wg-lacZ reporter expression is seen all along the ventral polar
margin. Dashed lines in D,E mark the boundary between the dorsal- and the
ventral-half of the eye. (D,E) Inset shows wg-lacZ expression (red)
in a separate channel in second instar eye imaginal discs. (F) In the
second instar eye disc, LOF clones of L cause ectopic induction of Wg
and Drice in the ventral eye (arrowhead). Separate channels are shown in
inset.
|
