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Figure 6


Fig. 6. The E13.5 p63-/--bladder primary cell cultures were transfected with adenoviruses expressing GFP, TAp63{gamma}, {Delta}Np63ß and {Delta}Np63{gamma}. The tensiometry of Bax, Apaf1 and ß-actin bands was recorded. The ratios of Bax/ß-actin and Apaf1/ß-actin of the specimens were compared with that of the GFP-infected controls (assigned to be 100). (A,B) Relative Bax/ß-actin (A) and Apaf1/ß-actin (B) ratios (Student's t-test, *P<0.05, **P<0.01). (C,D) Immunohistochemical staining of p53 in E11.5 wild-type (C) and p63-/- (D) UGS (200x). Arrows represent the ventral UGS. (E,F) Immunohistochemical staining of p73 in E11.5 wild-type (E) and p63-/- (F) UGS (100x). (G,H) The qPCR relative expressions of p53 (G) and p73 (H) in E12.5 and E13.5 wild-type (WT) and p63-/- bladders.





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