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Figure 2


Fig. 2. Expression of Bhlhb5 in GABAergic amacrine and OFF-cone bipolar subtypes. Sections from P28 mouse retinas were doubleimmunolabeled with anti-Bhlhb5 (red) and subtype-specific markers (green) as indicated. (A-C) The Bhlhb5+ cells in the GCL and the ACL are Pax6+ amacrine cells. (D-F) Bhlhb5 is expressed in GAD65+ GABAergic amacrine cells. (G-I) Bhlhb5 is co-expressed with Prox1 in displaced amacrine cells (arrows) in the GCL and bipolar cells (arrowheads) in the INL. (J-L) Bhlhb5 is co-expressed with Chx10 in bipolar cells (arrowheads). Inserts show the double-labeling of bipolar cells (asterisks) at high magnification. (M-O) All Bhlhb5+ bipolar cells are Vsx1+ cone bipolar cells (white arrowheads). (P-R) All Bhlhb5+ bipolar cells (white arrowheads) are recoverin+ Type 2 OFF-bipolar cells; red arrowheads indicate the OFF-bipolar cells immunoreactive to recoverin only. Scale bar: 50 µm.





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