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Figure 2


Fig. 2. Mesoderm induction by FoxD3. At the one-cell stage, embryos were injected in the animal pole with 200 pg of Xenopus FoxD3 (xFoxD3) or mouse FoxD3 (mFoxD3), explants were prepared at the late blastula stage (stage 9), and explants were analyzed for morphogenesis, tissue differentiation and gene expression. At the tailbud stage (stage 25), convergent extension movements were observed in response to xFoxD3 and mFoxD3 (A-C), and differentiated somitic muscle was detected in the FoxD3-expressing explants (D-F) using a muscle-specific antibody (12/101). (G-I) Explants stained with 12/101 were sectioned and counterstained (H&E) to show the presence of somitic muscle (sm), notochord (nc) and neural tube (nt) in FoxD3-induced explants. (J) Gene expression in explants was examined by RT-PCR for Brachyury (Xbra), Goosecoid (Gsc) and Xwnt8 at the midgastrula stage (stage 11), and for Muscle Actin (M. Actin) and NCAM at the tailbud stage (stage 25). EF1{alpha} is a control for RNA recovery and loading, intact embryos (Embryo) served as a positive control and an identical reaction without reverse transcriptase controlled for PCR contamination (Embryo-RT).





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