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Fig. 5. Mesodermal gene expression is dependent on FoxD3 function.
(A-F) Control. At the four-cell stage, each blastomere was injected in
the marginal zone with 500 pg of VP16-FoxD3 RNA (G-L), 25 ng of FoxD3MO
(M-R), or 25 ng of mismatch MO (S-X). At the early gastrula
stage (stage 10.25), embryos were analyzed by in situ hybridization for the
expression of the indicated genes. The results shown are representative of
three independent experiments (n=12-18 embryos per sample in each
experiment). Vegetal views are shown for Brachyury, Chordin, Xwnt8,
Mixer and Opl, animal views are shown for Dlx3, and
dorsal is up for all panels.
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