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Figure 2


Fig. 2. EphB2 and ephrinB1 fusion proteins disrupt proper sympathetic ganglia formation. (A) E3 embryo schematic with EGFP-labeled trunk neural crest cells migrating through the rostral somite and dispersed adjacent to the dorsal aorta. The red oval indicates axial level and size of the fusion protein injection. (B) Magnified trunk region of the embryo where the inhibitor was injected, showing focal delivery the length of one somite (red oval). (C) IgG-Fc-control injected embryo (n=8) with normal sympathetic ganglia formed adjacent to the rostral somite. The horizontal grid indicates the rows where mean fluorescence intensity was calculated, and plotted onto the graph to the right. (D) EphB2-Fc injected embryos (n=6). Neural crest cells found along the anteroposterior axis of the dorsal aorta in a random orientation. Circles indicate the location of presumptive sympathetic ganglia. (E) ephrinB1-Fc injected embryo (n=6). Neural crest cells evenly distributed along the anteroposterior axis of the dorsal aorta. The red oval indicates the inhibitor injection site. Scale bar: 20 µm. c, caudal somite; r, rostral somite; sg, sympathetic ganglion.





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