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Fig. 5. Cell shape and velocity comparisons in neural crest cells transfected
with EGFP, DN-cadherin or FL-cadherin. Cells imaged at E3-3.5 during
sympathetic ganglion formation adjacent to the dorsal aorta. (A)
Control EGFP-expressing neural crest cells. (B) FL-cadherin-expressing
neural crest cell. (C) DN-cadherin-expressing neural crest cells.
(D) Graph of number of filopodia on cells expressing EGFP control,
3.6±0.79 (s.d.), FL-cadherin, 6.1±0.91 and DN-cadherin,
1.9±0.6. (E) Graph showing average cell velocities during
ventral migration and after reaching dorsal aorta. The schematic shows the
region within the embryo that cells were migrating through when the velocity
was measured. Ventral migration through the rostral somite for EGFP control,
50±12.1 µm/hour, FL-cadherin, 19.3±5.14 µm/hour, and
DN-cadherin, 24.7±4.63 µm/hour. Cell velocities after reaching
dorsal aorta, EGFP control 62.1±15.3 µm/hour, FL-cadherin,
16.2±2.4 µm/hour, and DN-cadherin, 29.2±3.5 µm/hour.
Asterisk and caret (D,E) indicate significance, P<0.01,
P<0.001, respectively. Scale bars: 10 µm in A-C; 20 µm in D.
c, caudal somite; r, rostral somite.
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