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Fig. 5. Moe forms a complex with Crumbs proteins, Nok (Pals1), and Has
(aPKC
). (A) Anti-panCrb (anti-CRB3) antibodies
coimmunoprecipitate Moe (recognized by guinea pig anti-Moe), Crb2a/b, and Nok
from adult eye. NRS, normal rabbit serum. Arrows indicate Moe, Crb2a/b, Nok
protein. One asterisk indicates rabbit antibody; two asterisks indicate
cross-reactivity of anti-guinea pig-HRP with rabbit antibody. (B)
His-Moe_FERM, but not His-LacZ, pulls down a 
150 kDa protein
from 3 dpf larval lysates that is recognized by anti-panCrb antibodies.
(C) His-Moe_FERM, but not His-LacZ, pulls down an 80 kDa
protein from wild-type 3 dpf larval lysates, but not from nok morphant
lysates, recognized by anti-Nok antibodies. (D) Anti-Nok antibodies
recognize an 80 kDa protein in 3 dpf wild-type lysates that is absent in
nok mutants and morphants in western blot. (E) His-Moe_FERM,
but not His-LacZ, pulls down an 80 kDa protein from adult eye
lysates recognized by anti-Nok antibodies. (F) His-Moe_FERM pulls down
75 kDa and 50 kDa proteins from 3 dpf larvae lysates that are
recognized by anti-PKC antibodies. (G) Anti-PKC
antibodies recognize proteins of approximately 75, 57, 54 and 50 kDa in
western blot of 3 dpf wild-type lysates; the 75 kDa and 50 kDa proteins are
absent in lysates from has mutants (Has-/-).
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