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Fig. 4. Expression of c-Kit and Steel Factor. (A) Anti-c-Kit staining
of E10.5 embryo slices shows that both midline (arrowheads) and lateral germ
cells express c-Kit protein (observe colocalization in overlay). (B)
Dissected midlines (M) have less than two-thirds steel expression
compared with bilateral (L) regions as determined by real-time RT-PCR.
(C) Isotype-matched primary antibody serves as a negative control for
c-Kit and Steel antibodies. (D) Immunostaining of a
Steel-/- embryo with an anti-Steel antibody demonstrates
the antibody's specificity (note the lack of red signal). (E) A
Steel wild-type embryo at E9.5 has Steel protein in the genital
ridges and midline structures, including the coelomic angles and tissues
ventral to the dorsal aorta. (F) At E10.75, Steel protein becomes
restricted bilaterally to the genital ridges. Ao, aorta. Scale bars: 100
µm.
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