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Figure 6


Fig. 6. Mapping the Steel (KitlSl) deletion. (A) The exact positions of the 5'- and 3'-breakpoints of KitlSl are indicated as boxed nucleotides, 1 (position 99,177,807) and 2 (position 100,151,173). The total deletion is 973,366 bp, not including an insert of four nucleotides. Nucleotide positions correspond to NCBI Map Viewer numbers. (B) A genotyping strategy was designed to identify wild-type and deleted alleles of Steel. (Top) The blue line represents DNA containing the Steel gene, and the dotted region represents the portion that is missing in the deleted allele. The red arrows represent primers (see Materials and methods). (Bottom) A gel showing a genotyping reaction using a mix of all three primers. Heterozygotes are identifiable by bands generated from both 1+2 and 1+3.





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