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Figure 2


Fig. 2 . Expression and structure of the snky transcript. (A,B) Northern blots probed with a genomic fragment that contained CG11281 detected a 2.7 kb transcript that was present in testis, absent in male carcasses (A) and reduced in size and abundance in snky1 testes compared to its parental strain (B). Bottom panel shows levels of rp49 mRNA in each lane as a loading control. (C) Transcript structure was determined by RT-PCR. Primers used for cDNA amplification are indicated by arrows. The transcript is contained within the AvaI genomic fragment, which was used as a probe for northern analysis. Fragment endpoints are labeled according to Drosophila Genome Project Release 4.0 coordinates. Transcription initiation site (+1) determined by 5' RACE is nucleotide 3L 13,131,973. Positions of the start and stop codons of the 2145 bp ORF (black box), the 72 bp intron and the predicted polyadenylation site are also indicated.





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