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Figure 6


Fig. 6. Localization of Snky-GFP and GFPsecr during fertilization. (A-D) GFP (green) was monitored in eggs fertilized by sperm from snky1 males that carried two copies of a snky-GFP transgene. Nuclei (red) were stained with DAPI. Insets to the right of each picture show higher magnification view of the GFP signal. (A) A single GFP signal is observed just apical to the condensed sperm nucleus and (B) more distantly as the sperm nucleus decondenses. (C) GFP is seen near the apposing male and female pronuclei and (D) by the chromosomes during prometaphase of the first embryonic cycle. (E-H) In eggs fertilized by sperm from males expressing GFPsecr, the GFP signals appear similar to those described for Snky-GFP. Scale bars: 3 µm in A,B,E,F; 6 µm in C,D,G,H; 2 µm in the higher magnification insets.





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