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Fig. 3. Specified, Myf5-positive myoblasts are drastically reduced in the Pitx2^null mutant branchial arch. (A,B) Lineage tracing with the Myf5^cre allele indicates that the Myf5 lineage is drastically reduced in the first branchial arch of 9.5 dpc Pitx2^null mutant embryos (arrows). (C,D) By 10.5 dpc, the Myf5 lineage has expanded in the wild-type first branchial arch (arrow in C) but is still greatly reduced in the Pitx2^null branchial arch. LacZ-positive cells are detectable dorsal to the branchial arch in both control and Pitx2^null embryos (arrow in D). Also the Myf5 lineage that contributes to extraocular muscle is mislocalized in Pitx2^null embryos (arrowhead in D). (E,F) In the 11.5 dpc embryo, a small number of Myf5^cre-labeled cells can be detected, indicating that a few specified myoblasts are present in the Pitx2^null mutant branchial arch (arrow in E,F). Abnormal localization of the EOM precursors is still observed in the Pitx2^null embryo (arrowhead in F). e, eye; fn, frontonasal mass; md, mandible; mx, maxilla; ov, otic vesicle.

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