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Fig. 2. Wg accumulates in the cells that express the ligand in srt^7E4 embryos. Stage-9 (A-C) and stage-13 (D-F) embryos oriented anterior up, posterior down and ventral or ventrolateral surface facing out. (A,D) Expression of Wg in wild-type embryos shows a low level of Wg in Wg-expressing cells, and numerous Wg punctate endocytic vesicles in the receiving cells at both stages of development. (B) Wg accumulates in expressing cells in non-paternally rescued embryos at stage 9. Notice that punctate vesicles are absent and Wg fills expressing cells. (C) Similar retention of Wg is observed in srt^7E4 paternally rescued embryos, although a few endocytic vesicles can be detected in receiving cells (arrowheads). (E) By stage 13, Wg has completely faded from the epidermal surface of nonpaternally rescued srt^7E4 embryos and can be detected only in neuroblasts. (F) In paternally rescued stage-13 srt^7E4 embryos, there is still a significant level of Wg within the cells that express it, suggesting that the Wg-Hh-feedback signaling loop is still intact. Wg vesicles are also detected (arrowheads). The difference in background between wild-type and srt^7E4 embryos is due to the higher laser intensity used to collect data from wild-type embryos, which is not required for the mutant. For a direct comparison of Wg levels between wild-type and srt^7E4 mutant tissues see Fig. 6. All images are projections of three optical sections acquired at 1 µm z-intervals, except E, which is a single optical section. Scale bar: 20 µm.

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