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Fig. 5. srt^7E4 mitotic clones result in Wg accumulation at the dorsoventral boundary and blocks the activation of targets in Wg receiving cells in the wing disc. The wild-type expression pattern for Wg (red; A) and its downstream targets Senseless (Sen, blue; B) and Achaete (Ac, red; J) in third instar wing imaginal discs. (C) Images A and B merged with wild-type Green Fluorescent Protein (GFP). (D-I) The effects of srt^7E4 homozygous mutant tissues on the expression of Wg and Sen at two different magnifications. (K-L) Anterior Ac expression. srt^7E4-homozygous-mutant cells are marked by the absence of GFP shown in merged images (F,I,L,N). Retention of Wg within Wg-expressing cells at the dorsoventral boundary blocks dorsal and ventral expression of Sen (E,H). Sen is expressed when GFP-positive (heterozygous) cells are present at the dorsoventral boundary, but does not require wild-type srt because many srt^7E4-homozygous cells are present (Senexpressing cells excluded from outlined region in E and H). Arrows in G and H show two srt^7E4-mutant cells that express high levels of Sen, but not Wg. Similarly, anterior Achaete expression also requires a wild-type srt in Wg-expressing cells at the dorsoventral boundary (K,L). (M-O) A projection of optical sections of a Wg-stained (red) third instar wing disc shows the dramatic difference in the presence of Wg within the cells that express it between srt^7E4-mutant (left side) and heterozygous tissue (right side). Homozygous mutant cells are identified by the absence of GFP (O). Punctate Wg endocytic vesicles (arrowheads) are present adjacent to Wg-expressing heterozygous cells and absent near srt^7E4-homozygous tissue. There also appears to be an expansion in the number of cells that express Wg at the dorsoventral boundary when srt function is absent. All wing discs are orientated dorsal up and anterior left. Images A-I are single optical sections focusing on the Sen-expressing nuclei. (J-O) Projection of eight optical sections acquired at 1 µm z-intervals. Scale bars: 20 µm.

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