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Fig. 6. Hh signaling in wing imaginal discs does not require wild-type
srt. (A-F) Expression of the ligand Hh is shown in red
(A,D) and Ci in blue (B,E) to mark the anterior compartment and
anterior-posterior compartment boundary. (G-I) The expression of a
sensitive anterior target of Hh in receiving cells, Patched (Ptc), is shown in
red. Absence of GFP marks srt7E4-mutant cells in the
merged images (C,F,I). The expression and localization of Hh in signaling
cells and receiving cells appears wild type in the mutant clones regardless of
whether Hh is synthesized in or received by srt7E4-mutant
cells. Heterozygous tissue along the anteriorposterior boundary is outlined in
A and D. Wildtype anterior Ptc expression is also unchanged in mutant clones
(G) regardless of the location of homozygous mutants cells relative to the
anteriorposterior boundary (H,I). Wing discs are orientated dorsal up and
anterior left. All images are projections generated from eight optical
sections acquired at 1 µm z-intervals, except G, which is a single optical
section. Scale bars: 20 µm.
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