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Figure 1


Fig. 1. Ndst1 expression during lens development. (A) Ndst1 is expressed in the developing eye. RNA in situ hybridization was performed on mouse embryonic sections. Lens placodes at E9.5 and lenses at E12.5 were both stained with a Ndst1 antisense probe. As a control, samples were incubated with a Ndst1 sense probe. The Pax6 antisense probe specifically stained the developing lens placode (LP) and optic vesicle (OV) at E9.5, and lens epithelium (LE) and retina (RE) at E12.5. (B) RT-PCR analysis of Ndst gene expression in the lens and retina at E12.5. At this stage, only Ndst1 (N1), Ndst2 (N2) and mitochondria ribosomal subunit L19 were detected in lens mRNA by RT-PCR, whereas all four Ndst genes were expressed in the retina. No signal was detected in the absence of reverse transcriptase (- RT). N3, Ndst3; N4, Ndst4.





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