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Figure 3


Fig. 3. Lens cell proliferation defects in Ndst1 mutants. (A) Homozygous Ndst1-knockout embryos and wild-type controls were stained for BrdU (red), and the nuclei were counterstained with Hoechst at the 24-, 30- and 34-somite stages. (B) Cell proliferation was quantitated as the ratio of BrdU-positive cells versus Hoechst-positives cells at different stages of development [26- to 29-somite (s) stages; 30- to 33-s stages; and 34- to 38-s stages]. There was a consistent reduction of cell proliferation in Ndst1-mutant lenses compared with wild type (Student's t-test: 26- to 30-somite stages, P<0.001; 30- to 34-somite stages, P<0.01; 34- to 38-somite stages, P<0.001. At least four embryos were analyzed for each genotype at each stage). (C) Lack of apoptosis defects in Ndst1-mutant lens vesicles. TUNEL staining in Ndst1 mutants was normal in the lens vesicle (arrows), but increased in periocular mesenchyme (arrowheads). KO/KO, homozygous Ndst1-knockout embryos; WT, wild type.





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