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Fig. 8. PCP pathways are impaired in Tbx20 mutants. (A-X)
Ret, Fzd7, Wnt11, Vang1, Vang2, Pk1, Dsh3 and Celsr3
expression in flat-mounted hindbrains or transverse sections at r4 from E11.5
Tbx20 mutants or littermate controls. To localize facial cells at r4,
Tbx20 and Hoxb1 probes were used on adjacent sections
(C,F,I,L, and see Fig. S6 in the supplementary material). (A-F) Ret
is ectopically expressed at r4 in Tbx20 mutants. (G-R) Fzd7,
Wnt11, Vang1 and Vang2 are absent at r4 in Tbx20
mutants, in constrast to controls. (S-X) Pk1 expression is reduced in
Tbx20 mutants whereas Dsh3 and Celsr1 are
unchanged. The boundaries of r4 are indicated by the white dashed lines; the
blue dashed line encircles facial cells. (Y) Summary of genes examined
in facial neurons from controls and Tbx20 mutants. Each bar
represents the expression of an individual gene from r4 to r6. Striped bars
represent genes expressed by facial neuron progenitors residing at r4. Genes
expressed at similar stages are aligned next to each other. (Z) Summary
of the genetic cascade required (but not necessarily sufficient) for facial
motor neuron development and migration based on data presented here and
elsewhere (reviewed by Chandrasekhar,
2004). It is unknown whether Tbx2 is required to activate PCP gene
expression. Scale bars: in J, 400 µm for A,D,G,J; in F, 250 µm for
B,C,E,F; in X, 110 µm for H,I,K-X.
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