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Figure 1


Fig. 1. Coexpression of eed and Bmi1 in wild-type embryos. Representative images of flat-mount E8.5 embryos (A,E) and sectioned E12.5 embryos (B,F) mRNA in situ hybridization with eed (A,B) and Bmi1 antisense cRNA probes (E,F). Note coexpression of eed and Bmi1 mRNA in somites (arrowheads in A,E) and prevertebrae (arrowheads in B,F), as well as neuroectoderm (arrows in A,B,E,F). Control hybridization with eed and Bmi1 sense cRNA probes revealed no specific signal on sectioned E12.5 embryos (C,G). Immunohistochemistry detected nuclear expression of EED and BMI1 in E12.5 prevertebrae (D,H). The images were captured at 20x (B,C,F,G), 50x (A,E), and 1000x (D,H).





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