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Fig. 4. Tlr and Tld process the pro-peptides of several TGF-ß
ligands in Drosophila. (A) Alignment of predicted
BMP-1/Tolloid cleavage sites in Act, Daw, Myo and Mav pro-peptides.
*, residues that were changed to alanine in the mutated forms of
the ligands shown in F; red arrow, cleavage site. (B) Schematic of
tagged pro-peptide and pro-peptide fragments visible by western analysis.
(C-F) Conditioned media from S2* cells co-transfected with
ligands and enzymes as indicated were analyzed by western blotting using
anti-V5 antibody for detection of N-tagged pro-peptides and cleaved fragments.
Tm designates the corresponding mutant in which the BMP-1/Tolloid site was
destroyed.
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