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First published online November 21, 2006

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Ldb1 changes partners for haematopoiesis

During haematopoiesis, coordinated changes in transcription drive the differentiation of haematopoietic stem cells. Now Frank Grosveld and colleagues suggest that the LIM domain binding protein Ldb1 facilitates these transcriptional changes by forming distinct complexes with different partners (see p. 4913). Ldb1 is a cofactor for several DNA-binding proteins, including haematopoietic transcription factors. To find new Ldb1 binding partners, the researchers expressed biotin-tagged Ldb1 in C88 erythroleukaemic cells and pulled out interacting proteins with streptavidin-coated beads. New partners included the repressor protein Eto-2, the cyclin-dependent kinase Cdk9, and the bridging factor Lmo4. The researchers show that the composition of Ldb1 complexes changes during the differentiation of C88 cells. They also report that these Ldb1 partners are essential for definitive haematopoiesis in zebrafish and are coexpressed in pre-haematopoietic cells in mouse embryos. Finally, the researchers speculate that changes in Eto-2 and Lmo4 levels cause a shift from a large Ldb1-containing complex to a smaller complex that is required for haematopoiesis.

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Related articles in Development:

Novel binding partners of Ldb1 are required for haematopoietic development

Natalia Meier, Sanja Krpic, Patrick Rodriguez, John Strouboulis, Maria Monti, Jeroen Krijgsveld, Martin Gering, Roger Patient, Arnd Hostert, and Frank Grosveld
Development 2006 133: 4913-4923. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?