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Fig. 1. lsn-mutant phenotype. (A,B) Lateral views of live larvae at 96 hpf, showing the abnormal development of the jaw, eye and heart in lsn (B) when compared with wild type (A), and lack of swim bladder in lsn. Arrow in A indicates the swim bladder. (C,D) Lateral views of 96 hpf embryos stained with anti-Hu antibody shows normal DRG development in lsn but a reduced number of enteric neurons that are absent from the distal end of the gut tube. Stars indicate the end of the gut tube; arrows indicate DRGs; arrowheads indicate enteric neurons. (E,F) Lateral views of the gut tube of 96 hpf embryos stained with anti-Hu antibody showing reduced number of neurons in lsn.





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