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Fig. 1. Hh-Np activity defines the limit of serrate expression in the ventral ectoderm. In all panels anterior is towards the left. (A-A'') Wild-type embryos. (B-B'') hh^AC, engal4 UAS-hh-N embryos. (C) hh^AC, engal4 UAS-hh-N-CD2 embryos. (D) disp and (E) ttv germline clone embryos. In A-C, ser mRNA is in red, Hh protein is in green and Wg protein is in blue; in D,E, ser mRNA is in blue and En protein is in brown. (A-A'') In wild-type stage 11 embryos, the Hh-Np gradient is symmetric, when compared with the asymmetric Wg gradient. Hh-Np LPSs are detected at a distance of three or four cells from their source (arrows in A'), and very few Hh-Np LPSs are detected within the ser expression domain (outline in A-A''). No LPS-like structures are detected in Hh-N-expressing embryos (B,B'), and ser is expressed broadly and juxtaposed with the Hh-N source (arrows in B-B'' indicate the boundary between Hh-N- and ser-expressing cells). (C) When Hh-N-CD2 replaces endogenous Hh, ser is no longer repressed. (D,E) In the absence of any disp or ttv function, ser expression is no longer repressed. Derepression of ser expression is observed in ttv mutant to a lesser extent than in hh or disp mutants. This could be due in part to the remaining rho expression, which is independent of Ttv activity and of Hh-LPS movement (Gallet et al., 2003).

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