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Fig. 6. Short-range activity of Hh-N in columnar cells. dpp-lacZ
(red) expression in wild-type wing imaginal disc (A) or around clones
of cells (marked by GFP in green) expressing either Hh-Np (B-D),
Hh-N (E-G) or Hh-N-CD2 (H-J) produced in columnar
cells using the flip-out technique. (D,G,J) Confocal z-sections
(apical side upwards). Clones of cells expressing Hh-Np in the anterior
compartment of the disc induced dpp expression in a six- or
seven-cell wide region around the clone (B-D), while Hh-N was able only to
activate dpp expression in a three- or four-cell region around the
clone, even when the clones were bigger than Hh-Np expressing clones (E-G).
Hh-N-CD2 expressing clones activated dpp in 1 row of cells around the
clone (H-J).
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