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Fig. 1. Targeted disruption of the Cdx4 gene. (A) The
gene-targeting vector inserts an antisense floxed
PGK-hygror cassette into an EcoRV site upstream
of exon 2 and an antisense flipped PGK-neor cassette,
including a single loxP site into an EcoRI site downstream of exon 3,
resulting in a conditional allele of Cdx4
(Cdx4c). Cre-mediated deletion of the homeodomain in exon
2 and exon 3 generates the Cdx4-null allele
(Cdx4-). LoxP and FRT sites are represented by closed and
open red triangles, respectively. HIII, HindIII; KI, KpnI;
RI, EcoRI; RV, EcoRV; SI, SpeI. (B) Southern
blot analysis of male ES cell clones with a unique 5' genomic probe
reveals a 17 kb KpnI fragment generated by the targeted conditional
allele. A 3' internal probe reveals correct 3' targeting by
identification of a 17 kb KpnI fragment. (C) Histological
evaluation of the digestive tract of adult Cdx4-null and control
(wild-type) mice. Ki67 immunohistochemistry shows normal proliferation in the
intestinal and colonic crypts (brown cells). Unlike previous findings in
Cdx2+/- mice, no intestinal polyp-like lesions were
observed.
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