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Fig. 3. A subset of Cdx2+/-/Cdx4-/0 mutant embryos fails to undergo chorio-allantoic fusion. (A) Lateral view of freshly dissected E9.5 wild-type embryo. The allantois has fused to the chorionic part of the placenta (arrow). (B) Lateral view of a E9.5 Cdx2+/-/Cdx4-/0 compound mutant littermate that is defective in chorio-allantoic fusion (arrow). (C) Frontal view of the embryo shown in B. The allantois appears to end as a massive ball of cells (arrow). (D) Histological analysis of the allantois shown in C. Several allantoic vessels, containing nucleated embryonic blood cells (arrowheads) and the outer mesothelial layer (arrow) are visible. Pl, placenta; TB, tail bud. Scale bar: 100 µm.





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