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Fig. 6. Cdx expression and early vascular development in the allantois.
(A-C) In situ hybridization on E7.5 embryo sections reveals strong
expression of Cdx1 in the primitive streak and weak expression at the
base of the allantois (arrowhead in A). Expression is absent in the chorion
(asterisk in A). (B) Cdx2 is strongly expressed in the primitive
streak and in the outgrowing allantois (arrowhead) and in the chorion
(triangle). (C) Cdx4 expression is restricted to the primitive streak
and allantois (arrowhead), and is absent in the chorion (asterisk).
(D,E) Flk1 staining marks endothelial precursors that are present
throughout the E7.5 wild-type (D) and
Cdx2+/-/Cdx4-/0 mutant allantois (E). The
allantois of the wild type was slightly smaller than that of the compound
mutant, explaining the lower Flk1 staining in D. (F-I) Pecam
staining of allantoises isolated at E8.25 just after chorio-allantoic fusion.
Primary endothelial networks in wild-type (F and H) and
Cdx2+/-/Cdx4-/0 mutant (G and I) allantoises
are visible. The wild-type E8.25 allantois in F shows organization of
endothelial cells into vessels with numerous branching and connecting vessels
(arrowheads). Endothelial cells are less well organized in
Cdx2+/-/Cdx4-/0 compound mutant littermate
shown in G, with clumps of Pecam-positive cells (arrows). The
Cdx2+/-/Cdx4-/0 allantois in I is, in this
case, smaller than the wild-type control (H). Scale bars: 200 µm in A-C; 40
µm in D,E; 20 µm in F,G; 80 µm in H,I. D, distal; P, proximal.
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