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Fig. 6. Temporal regulation of svp mRNA expression. In situ hybridisation for svp mRNA (magenta, A-C',E-G') combined with anti-Eg (green, A-C) and anti-Hb (green, E-G), and double staining with anti-Eg (green, D) and anti-Svp (magenta, D,D'). NBs and their GMCs are outlined. (A,B) Wild type. NB6-4T expresses svp before it undergoes the first mitosis (arrowhead in A,A'). After mitosis, svp expression stops immediately (6-4T cells in B,B'). Likewise, NB7-3 expresses svp before its first divison (B,B', arrow). NB2-4T generates one GMC (small 2-4T cell in A,A') before it expresses svp (curved arrow in B,B'). There is no detectable svp mRNA within GMC2-4Ta (B,B'). NB3-3 and its progeny do not express svp mRNA at these early stages. (C) stg⁴. At late stage 11, NB6-4T, 2-4T and 7-3 still express svp mRNA in cell cycle-arrested embryos, indicating that svp expression without cell division is not sufficient to switch off hb expression. (D) Even at early stage 12 there is hardly any Svp protein detectable in NB2-4T, 6-4T and 7-3 in stg⁴ mutant NBs. (E,F) Wild type. NB7-1 (identified by position and En expression, not shown) is initially negative for svp mRNA (E,E'). svp expression starts after the generation of its first GMC (F,F'). (G) stg⁴. Like NB2-4T, NB7-1 also starts to express svp mRNA at normal time, even without the generation of its first GMC. Scale bar: 20 µm.

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