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Fig. 10. Single chromogen whole-mount staining of ecmAO: lacZ expression
in migrating slugs. dimB- and a control (random integrant) strain
expressing the ecmAO:lacZ reporter fusion were developed for 24 hours in a
slug migration chamber. The slugs were then fixed and stained to detect
ß-galactosidase. The staining times were controlled, so as to produce
approximately equal intensity staining in the extreme tip of the control and
the null mutant. However, the same difference in staining patterns was
observed at a variety of staining times up to 30 minutes, at which time the
reactions started to plateau.
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