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Fig. 8 Genetic analysis of DimB function. (A) Isolation of
dimB-mutants. A mixture of putative dimB disruptants (marked
with an asterisk) and random integrants (i.e. clones where the disruption
vector integrated at a position other than the dimB locus) was
analysed by western transfer with the C terminus-specific DimB antibody. The
lane on the left contains DimB:HIS fusion protein and those on the right were
loaded with Ax2 total protein isolated at the slug stage. (B)
Morphological and behavioural phenotypes of the dimB- mutant. (Top) A
dimB- and a control (random integrant) strain were developed on water
agar to the slug stage. (Bottom) A dimB- and a control (random
integrant) strain were developed on water agar in a darkened chamber with a
narrow slit for 48 hours. The slugs were then lifted, with their adherent
trails, onto a clear plastic sheet which was stained with Coomassie Blue.
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