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Fig. 4. dimB- exhibits gene expression and cell-autonomous
defects. (A) Expression pattern of representative prestalk and
prespore markers at the slug stage of development. The prestalk marker
ecmAO/lacZ shows a reduced zone of expression compared with wild
type. The prespore marker cotB/lacZ shows an expanded zone of
expression, although expression is weaker in the expanded region than in the
prespore zone proper. (B) The dimB- mutant exhibits
cell-autonomous defects when mixed with wild-type cells. A minority (2%) of
wild-type or dimB- mutant cells were labelled by
constitutive expression of lacZ and mixed with unlabelled cells.
Wild-type and dimB- mutant cells are evenly distributed in
homotypic control chimaeras (a and d). dimB- mutant cells
preferentially localize to the rear of the prespore zone when mixed with an
excess of wild-type cells (b). Wild-type cells localize to the pstO and
prespore zone when mixed with dimB- mutant cells (c).
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