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Fig. 5. dimB- mutant cells fail to induce prestalk markers
and stalk cells in response to DIF-1. (A) Stalk cell induction was
measured in response to DIF-1 in the cAMP removal assay. The assay was
repeated at least three times and a representative example is shown. Wild-type
cells (AX4) differentiated as stalk cells, whereas mutants defective either in
dimA or in dimB, or in both (dimAB-)
failed to do so. (B) In the dimB- mutant, a
prestalk reporter (ecmAO/lacZ) was non responsive to DIF-1, whereas a
prespore marker (cotB/lacZ) was repressed as in the wild type. The
results are means of three experiments where each assay was performed in
triplicate. (C) The dimB- mutant did not show rapid
induction of prestalk gene expression (ecmA or ecmB) in
culture, although the prespore gene (cotB) was repressed. After
treatment with cAMP for 9 hours, samples were incubated with or without DIF-1
for 3 hours. Expression levels were normalized to IG7 and compared with or
without DIF-1. In the case of ecmA and ecmB, fold increase
is shown, and for cotB fold decrease. Broken line indicates no
change.
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